Document 3174
 DOCN  M94A3174
 TI    Latent infection of human T-cell leukemia type I (HTLV-I) in human
       monocytic cell (THP-1).
 DT    9412
 AU    Ohara Y; Iwasaki Y; Terunuma H; Tsunoda I; Mizobuchi M; Department of
       Neurological Sciences, Tohoku University School of; Medicine, Sendai,
       Japan.
 SO    Int Conf AIDS. 1994 Aug 7-12;10(1):136 (abstract no. PA0164). Unique
       Identifier : AIDSLINE ICA10/94369401
 AB    OBJECTIVE: Besides CD4-positive lymphocytes, monocyte/macrophage (M/M)
       lineage cells have been shown to be infected with HTLV-I. Moreover, in
       the rat CNS disease induced by inoculation of HTLV-I carrying lymphoid
       cells, cellular infiltrates in the CNS are exclusively mononuclear cells
       of M/M lineage. For further elucidation of the role of M/M in HTLV-I
       infection, we established HTLV-I infected clones from a human monocytic
       cell line (THP-1). METHODS: 1 x 10(5) cells of THP-1 cells were
       cocultured with an equal number of MT-2 cells treated with mitomycin C
       in RPMI 1640 medium supplemented with 10% fetal calf serum. The
       propagating cells were serially passaged twice a week. After about three
       months, cells were cloned by limiting dilution. 7 clones with positive
       HTLV-I signals were then obtained. The integration of HTLV-I were
       examined with both Southern blot and PCR. Expression of viral messages
       or antigen was also examined by RT-PCR or flowcytometry. RESULTS AND
       DISCUSSION: HTLV-I proviral DNA was monoclonally integrated, and message
       of either pX or env was hardly detected even with RT-PCR. However, an
       overnight culture of the clone in the supernatant of MT-2 cells induced
       the expression of viral messages. Therefore, THP-1 cells are thought to
       be latently infected with HTLV-I. And this cloned cell line might be
       useful to study the relationship between infected lymphocytes and M/M.
 DE    Blotting, Southern  Cell Line  Clone Cells  Culture Media  DNA,
       Viral/ANALYSIS  Flow Cytometry  Gene Expression  Genes, env  Human
       HTLV-I/GENETICS/*PHYSIOLOGY  Mitomycin C/PHARMACOLOGY  Monocytes
       Polymerase Chain Reaction  Proviruses/GENETICS/PHYSIOLOGY  RNA,
       Messenger/BIOSYNTHESIS  *Virus Integration  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).